E2F1 antibody (acLys120)

Catalog No. ABIN4901413

This Rabbit Polyclonal antibody specifically detects E2F1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for E2F1 antibody (acLys120) (ABIN4901413)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F1 Antibody

Binding Specificity: AA 100-170, acLys120

Purpose: E2F-1 is a protein encoded by the E2F1 gene which is approximately 46,9 kDa. E2F-1 is localised to the nucleus. It is involved in glioma, cyclins and cell cycle regulation and activation of BH3-only proteins. It plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. E2F-1 is expressed in the skin, pancreas and the nervous system. Mutations in the E2F1 gene may result in retinoblastoma pharyngoconjunctival fever. STJ97800 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of E2F-1 (Acetyl Lys120).

Specificity: The antibody detects endogenous E2F-1 (Acetyl-Lys120) protein

Characteristics: Rabbit Polyclonal to E2F-1 (Acetyl-Lys120).

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthetic peptide from AA range: 100-170.

Isotype: IgG

Application Details

Application Notes: WB 1:5000-10000 ELISA 1:10000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS,  pH 7.4, containing 0.02 % sodium azide as Preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F-1

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy