E2F1 antibody (acLys125)

Catalog No. ABIN4901417

The Rabbit Polyclonal anti-E2F1 antibody is suitable to detect E2F1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for E2F1 antibody (acLys125) (ABIN4901417)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F1 Antibody

Binding Specificity: acLys125

Purpose: E2F-1 is a protein encoded by the E2F1 gene which is approximately 46,9 kDa. E2F-1 is localised to the nucleus. It is involved in glioma, cyclins and cell cycle regulation and activation of BH3-only proteins. It plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. E2F-1 is expressed in the skin, pancreas and the nervous system. Mutations in the E2F1 gene may result in retinoblastoma pharyngoconjunctival fever. STJ98840 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of E2F-1 (Acetyl-K125).

Specificity: This antibody detects endogenous levels of Histone H3 (Di-Methyl-K80). It doesn't reacte with total or Tri-Methy or Mono-Methyl protein.

Characteristics: Rabbit Polyclonal to Histone H3 (Di-Methyl-K80).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized Tri-Methyl peptide derived from Human Histone H3

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
ELISA 1:5000-20010

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F-1

Gene ID: 8350

UniProt: P68431

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy