ERK1 antibody

Catalog No. ABIN4901491

This Mouse Monoclonal antibody specifically detects ERK1 in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERK1 antibody (ABIN4901491)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5E9

Product Details anti-ERK1 Antibody

Purpose: ERK1 is a protein encoded by the MAPK1 gene which is approximately 41,3 kDa. ERK1 is localised to the cytoplasm, cytoskeleton ad nucleus. It is involved in RET signalling, activated TLR4 signalling, the IL-2 pathway, regulation of lipid metabolism and insulin signalling-generic cascades. This protein falls under the MAP kinase family and acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Upon its activation, it translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. ERK1 is expressed in the cells of the nervous system, blood, lung, liver and skin. Mutations in the MAPK1 gene may result in small intestine neuroendocrine neoplasm. STJ97780 was developed from clone 5E9 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of MAPK3.

Specificity: ERK1 Mouse Monoclonal Antibody (5E9) detects endogenous levels of MAPK3

Characteristics: Mouse Monoclonal to ERK1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Recombinant peptide derived from ERK1

Isotype: IgG1

Application Details

Application Notes: IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins