ATG3 antibody

Catalog No. ABIN4906359

This anti-ATG3 antibody is a Mouse Monoclonal antibody detecting ATG3 in WB and IHC (p). Suitable for Human and Mouse.

Quick Overview for ATG3 antibody (ABIN4906359)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7C2

Product Details anti-ATG3 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein G.

Immunogen: This ATG3 antibody is generated from a mouse immunized with recombinant protein of human ATG3.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3

Background:

Synonyms: APG3, APG3L, PC3-96, APG3-LIKE, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, hApg3, Protein PC3-96, ATG3

Background: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene ID: 64422

UniProt: Q9NT62

Pathways: Autophagy