Cyclin D1 antibody (AA 61-110) (PE)

Catalog No. ABIN4908464

The Rabbit Polyclonal anti-Cyclin D1 antibody has been validated for WB and FACS. It is suitable to detect Cyclin D1 in samples from Human, Mouse, Rat, Cow, Rabbit and Sheep.

Quick Overview for Cyclin D1 antibody (AA 61-110) (PE) (ABIN4908464)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Cyclin D1 Antibody (PE)

Binding Specificity: AA 61-110

Cross-Reactivity: Cow, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background:

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1

Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Gene ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling