Lactate Dehydrogenase A antibody (N-Term)

Catalog No. ABIN492379

The Mouse Monoclonal anti-Lactate Dehydrogenase A antibody (Clone AT1A4) (ABIN492379) specifically detects Lactate Dehydrogenase A in WB, IF and EIA. The antibody is reactive with Human samples.

Quick Overview for Lactate Dehydrogenase A antibody (N-Term) (ABIN492379)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: AT1A4

Product Details anti-Lactate Dehydrogenase A Antibody

Binding Specificity: AA 1-332, N-Term

Specificity: The antibody recognizes Human LDHA. Other species not tested.

Characteristics: Synonyms: LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59, LDH muscle subunit,Cell proliferation-inducing gene 19 protein, Renal carcinoma antigen NY-REN-59

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant human LDHA (1-332aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1: 500 - 1: 8000). Immunofluorescence (1: 500 - 1: 8000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA

Background: Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59

Gene ID: 3939

UniProt: P00338

Pathways: Warburg Effect