ORAI3 antibody (C-Term)

Catalog No. ABIN6990753

Product Details anti-ORAI3 Antibody

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Binding Specificity: AA 200-250, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: ORAI3 antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.

Purification: ORAI3 Antibody is affinity chromatography purified via peptide column.

Immunogen: ORAI3 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3. The immunogen is located within amino acids 200 - 250 of ORAI3.

Isotype: IgG

Application Details

Application Notes: ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ORAI3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ORAI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Alternative Name: ORAI3 (ORAI3 Products)

Synonyms: ORAI3 antibody, TMEM142C antibody, 9930124N15 antibody, BC061259 antibody, Tmem142c antibody, RGD1306538 antibody, ORAI calcium release-activated calcium modulator 3 antibody, ORAI3 antibody, Orai3 antibody

Background: ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.

Molecular Weight: Predicted: 32 kDa

Observed: 34 kDa

Gene ID: 93129

UniProt: Q9BRQ5