PKC theta antibody (C-Term)

Catalog No. ABIN492623

The Mouse Monoclonal anti-PKC theta antibody is suitable to detect PKC theta in samples from Human and Rat. It has been validated for WB.

Quick Overview for PKC theta antibody (C-Term) (ABIN492623)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1C2

Product Details anti-PKC theta Antibody

Binding Specificity: C-Term

Specificity: This monoclonal antibody 1C2 recognizes Protein kinase C theta (PKC theta).

Characteristics: Synonyms: PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type, Protein kinase C theta type

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin derived from the C-terminal domain of PKC theta.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated MCF-7 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated MCF-7 cellsDescription: Cell lysate from untreated MCF-7 cells, breast adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved MCF-7 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS with 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PRKCQ / PRKCT

Background: Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. The protein kinase C family of Ser/Thr kinases consists of 11 isoenzymes. PKC theta is a autophosphorylated protein kinase, a member of the novel PKC (nPKC) subfamily. PKC theta is expressed in most tissues.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta

Gene ID: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling