RICTOR antibody

Catalog No. ABIN492626

The Mouse Monoclonal anti-RICTOR antibody is suitable to detect RICTOR in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for RICTOR antibody (ABIN492626)

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RICTOR antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1G11

Product Details anti-RICTOR Antibody

Specificity: Recognizes Rictor.

Characteristics: Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin derived from Rictor Sequence.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Alternative Name: RICTOR

Background: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

Gene ID: 253260

UniProt: Q6R327

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events