Recombinant Keratin Acidic (AE1) antibody

Catalog No. ABIN4949246

This anti- antibody is a Rabbit Monoclonal antibody detecting in IF, WB, FACS and IHC (p). Suitable for Human.

Quick Overview for Recombinant Keratin Acidic (AE1) antibody (ABIN4949246)

Target: Keratin Acidic (AE1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRTL-1577R

Product Details

Purification: Protein A affinity chromatography

Immunogen: An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.

1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
3. Required HIER: boil tissue sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (3),Prediluted IHC only format: incubate for 30 min at RT (2),FACS: 0.5-1 μg/million cells in 0.1ml,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Acidic Cytokeratin

Background: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]