Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Keratin Acidic (AE1) antibody

AE1 Reactivity: Human, Rat, Chicken, Mouse, Rabbit, Monkey, Cow, Dog, Turtle IF, WB, FACS, IHC (p) Host: Mouse Monoclonal AE1 unconjugated
Catalog No. ABIN3024694
  • Target See all Keratin Acidic (AE1) Antibodies
    Keratin Acidic (AE1)
    Reactivity
    • 31
    • 10
    • 8
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    Human, Rat, Chicken, Mouse, Rabbit, Monkey, Cow, Dog, Turtle
    Host
    • 17
    • 11
    • 3
    Mouse
    Clonality
    • 31
    Monoclonal
    Conjugate
    • 14
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Un-conjugated
    Application
    • 23
    • 23
    • 23
    • 20
    • 11
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G purified
    Immunogen
    Solubilized keratin extract from human stratum corneum
    Clone
    AE1
    Isotype
    IgG1
    Top Product
    Discover our top product AE1 Primary Antibody
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Keratin Acidic (AE1)
    Alternative Name
    Acidic Cytokeratin (AE1 Products)
    Background
    Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails.  There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.

    This antibody is specific for the 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), and 40  kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

    Gene ID
    3858
You are here:
Support