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Keratin Acidic (AE1) antibody

AE1 Reactivity: Human, Rat IF, WB, FACS, IHC (p) Host: Mouse Monoclonal KRTL-1077 unconjugated
Catalog No. ABIN3023883
  • Target See all Keratin Acidic (AE1) Antibodies
    Keratin Acidic (AE1)
    Reactivity
    • 31
    • 10
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Human, Rat
    Host
    • 17
    • 11
    • 3
    Mouse
    Clonality
    • 31
    Monoclonal
    Conjugate
    • 14
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Un-conjugated
    Application
    • 23
    • 23
    • 23
    • 20
    • 11
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Characteristics
    This mAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.
    Clone
    KRTL-1077
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product AE1 Primary Antibody
  • Application Notes
    Optimal dilution of the Acidic Cytokeratin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Keratin Acidic (AE1)
    Alternative Name
    Acidic Cytokeratin (AE1 Products)
    Background
    This mAb recognizes the 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 40  kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40  kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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