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Recombinant Keratin Acidic (AE1) antibody (CF®488A)

AE1 Reactivity: Human IHC, IF, WB, FACS Host: Rabbit Monoclonal KRTL-1577R CF®488A Recombinant Antibody
Catalog No. ABIN6178766
  • Target See all Keratin Acidic (AE1) Antibodies
    Keratin Acidic (AE1)
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 31
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Human
    Host
    • 18
    • 10
    • 3
    Rabbit
    Clonality
    • 31
    Monoclonal
    Conjugate
    • 15
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    CF®488A
    Application
    • 23
    • 23
    • 23
    • 19
    • 12
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
    Purpose
    Recombinant Rabbit Monoclonal anti-Cytokeratin, Acidic (KRTL/1577R), CF488A Conjugate
    Characteristics
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human KRT77 protein fragment
    Clone
    KRTL-1577R
    Isotype
    IgG kappa
    Top Product
    Discover our top product AE1 Primary Antibody
  • Application Notes
    Immunohistology (formalin) 1-2 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Skin, Squamous cell carcinoma (SCC)

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Keratin Acidic (AE1)
    Alternative Name
    Cytokeratin, Acidic (AE1 Products)
    Background
    K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B
    Molecular Weight
    56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)
    Gene ID
    374454, 334989
    UniProt
    Q7Z794
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