HLA-C antibody

Catalog No. ABIN4951309

The Rabbit Polyclonal anti-HLA-C antibody is suitable to detect HLA-C in samples from Human, Mouse and Rat. It has been validated for WB, ICC and IHC (p).

Quick Overview for HLA-C antibody (ABIN4951309)

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-C antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HLA-C Antibody

Purification: Antigen affinity

Immunogen: Amino acids EYWDRETQKYKRQAQADRVNLRKLR of human HLA-C were used as the immunogen for the HLA-C antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HLA-C antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,ICC: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the HLA-C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HLA-C

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Alternative Name: HLA-C

Background: HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

UniProt: U5YBW8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response