PLK1 antibody (AA 86-430)

Catalog No. ABIN4952193

This Rabbit Polyclonal antibody specifically detects PLK1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PLK1 antibody (AA 86-430) (ABIN4952193)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLK1 Antibody

Binding Specificity: AA 86-430

Purification: Antigen affinity

Immunogen: Amino acids 86-430 of human PLK1 were used as the immunogen for the PLK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLK1 antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PLK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

UniProt: P53350

Pathways: Cell Division Cycle, M Phase