KEAP1 antibody (AA 422-449)

Catalog No. ABIN3031533

Product Details anti-KEAP1 Antibody

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Binding Specificity: AA 422-449

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Expected species reactivity: Pig

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 422-449 from the human protein was used as the immunogen for this KEAP1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the KEAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the KEAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1 (KEAP1 Products)

Background: KEAP1 acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome [UniProt]

UniProt: Q14145

Pathways: Maintenance of Protein Location