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SMAD2 antibody (AA 198-232)

The Rabbit Polyclonal anti-SMAD2 antibody is suitable to detect SMAD2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. (SMAD2 antibody (AA 198-232))

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Catalog No. ABIN3032615

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Quick Overview for SMAD2 antibody (AA 198-232) (ABIN3032615)

Target

See all SMAD2 Antibodies

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This SMAD2 antibody is un-conjugated

Application

Western Blotting (WB), ELISA

Discover our top product SMAD2 Primary Antibody

Binding Specificity
57

53

39

34

30

25

22

21

9

8

7

6

6

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 198-232

Purpose

SMAD2 Antibody

Predicted Reactivity

Bovine,Pig,Chicken

Purification

Antigen affinity

Immunogen

This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids of mouse Smad2.

Isotype

Ig Fraction
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3G9 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, Co-IP Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibody
SMAD2 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4D10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibody
SMAD2 Reactivity: Human WB, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibody
SMAD2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 83-264) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer250) antibody
KD Validated SMAD2 Reactivity: Human WB, FACS Host: Rabbit Monoclonal 23GB4150 unconjugated Recombinant Antibody

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 2D7 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 83-264) antibody
SMAD2 Reactivity: Human WB, IHC, FACS Host: Mouse Monoclonal 3C4 unconjugated

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles

Storage

4 °C,-20 °C

Storage Comment

Store at 4oC for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Target

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name

SMAD2

Background

SMAD2 is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. [UniProt]

UniProt

Q62432

Pathways

Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus