PKM2 antibody

Catalog No. ABIN4953284

This anti-PKM2 antibody is a Mouse Monoclonal antibody detecting PKM2 in WB, IF and ICC. Suitable for Human, Mouse, Rat and Primate.

Quick Overview for PKM2 antibody (ABIN4953284)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Mouse, Rat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1A7-G6-H6

Product Details anti-PKM2 Antibody

Purification: Protein G affinity

Immunogen: A human recombinant partial protein was used as the immunogen for this PKM2 antibody.

Isotype: IgG1

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:400

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Storage: -20 °C

Storage Comment: Store the PKM2 antibody at -20°C.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: Pyruvate Kinase / PKM2

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]

UniProt: P14618

Pathways: Warburg Effect