This recombinant H2A.ZK4ac antibody reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A
Purification
Protein A purified from animal origin-free supernatant
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4) was used as the immunogen for this recombinant H2A.ZK4ac antibody.
H2AFZ
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant H2A.ZK4ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 1-2 μg/mL
Restrictions
For Research Use only
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant H2A.ZK4ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
H2AFZ
(H2A Histone Family, Member Z (H2AFZ))
Alternative Name
Histone H2A.Z
Background
This recombinant H2A.ZK4ac antibody reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A