The Rabbit Monoclonal anti-H2AFZ antibody (Clone RM222) (ABIN4953320) specifically detects H2AFZ in WB, ELISA and ICC.
The antibody is reactive with All Species samples.
This recombinant H2A.ZK7ac antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
Purification
Protein A purified from animal origin-free supernatant
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7) was used as the immunogen for this recombinant H2A.ZK7ac antibody.
H2AFZ
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant H2A.ZK7ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 1-2 μg/mL
Restrictions
For Research Use only
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant H2A.ZK7ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
H2AFZ
(H2A Histone Family, Member Z (H2AFZ))
Alternative Name
Histone H2A.Z
Background
This recombinant H2A.ZK7ac antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.