Recombinant Histone H2B antibody (acLys20)

Catalog No. ABIN4953321

This Rabbit Monoclonal antibody specifically detects Histone H2B in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Histone H2B antibody (acLys20) (ABIN4953321)

Target: Histone H2B

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: RM235

Product Details anti-Histone H2B Antibody

Binding Specificity: acLys20

Characteristics: This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant H2BK20ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for Histone H2B

Target: Histone H2B

Background: This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Gene ID: 3018

UniProt: P33778