Recombinant SMAD4 antibody (C-Term)

Catalog No. ABIN4953422

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Antibody Type: Recombinant Antibody

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Characteristics: This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the C-terminus of human SMAD4 was used as the immunogen for this recombinant SMAD4 antibody.

Clone: RM277

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. IHC (FFPE): 1:1000-1:2000 (1),Western Blot: 1:500-1:1000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant SMAD4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Background: This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy