TLR4 antibody (AA 25-55)

Catalog No. ABIN3029171

The Rabbit Polyclonal anti-TLR4 antibody has been validated for WB and ELISA. It is suitable to detect TLR4 in samples from Mouse.

Quick Overview for TLR4 antibody (AA 25-55) (ABIN3029171)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TLR4 Antibody

Binding Specificity: AA 25-55

Purification: Purified

Immunogen: A portion of amino acids 25-55 from the mouse protein was used as the immunogen for this anti-TLR4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the anti-TLR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the anti-TLR4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: TLR4, a type I membrane protein that belongs to the Toll-like receptor family, cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response TLR4 Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR. TLR4 binds to LY96 via the extracellular domain, and to MyD88 and TIRAP via their respective TIR domains. The protein contains 19 leucine-rich (LRR) repeats, and It is highly expressed in heart, spleen, lung and muscle. Lower levels are found in liver and kidney. Interstrain analyses reveal that TLR4 is a polymorphic protein and that the extracellular domain is far more variable than the cytoplasmic domain, which is variable at the C-terminal.

UniProt: Q9QUK6

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins