ARF1 antibody (AA 80-106)

Catalog No. ABIN3029877

This anti-ARF1 antibody is a Rabbit Polyclonal antibody detecting ARF1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for ARF1 antibody (AA 80-106) (ABIN3029877)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ARF1 Antibody

Binding Specificity: AA 80-106

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Primate, Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 80-106 from the human protein was used as the immunogen for this ARF1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ARF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ARF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background: ARF1 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity, the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). [UniProt]

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process