Bcl-2 antibody (AA 75-110)

Catalog No. ABIN3030102

This anti-Bcl-2 antibody is a Rabbit Polyclonal antibody detecting Bcl-2 in WB, IHC, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Bcl-2 antibody (AA 75-110) (ABIN3030102)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 75-110

Cross-Reactivity (Details): Expected species reactivity: Bovine

Purification: Purified

Immunogen: A portion of amino acids 75-110 (BH3 binding domain) from the human protein was used as the immunogen for this Bcl-2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background: BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling