Cyclin D1 antibody (AA 267-294)

Catalog No. ABIN3029708

The Rabbit Polyclonal anti-Cyclin D1 antibody has been validated for WB and ELISA. It is suitable to detect Cyclin D1 in samples from Human.

Quick Overview for Cyclin D1 antibody (AA 267-294) (ABIN3029708)

Target: Cyclin D1 (CCND1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cyclin D1 Antibody

Binding Specificity: AA 267-294

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 267-294 from the human protein was used as the immunogen for this CCND1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CCND1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CCND1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling