Cyclin D3 antibody (AA 252-281)

Catalog No. ABIN3030598

Product Details anti-Cyclin D3 Antibody

Target: Cyclin D3 (CCND3)

Binding Specificity: AA 252-281

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Expected species reactivity: Bovine

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 252-281 from the human protein was used as the immunogen for this CCND3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CCND3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CCND3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Abstract: CCND3 Products

Background: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process