CDC25B antibody (AA 331-360)

Catalog No. ABIN3030451

This Rabbit Polyclonal antibody specifically detects CDC25B in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CDC25B antibody (AA 331-360) (ABIN3030451)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CDC25B Antibody

Binding Specificity: AA 331-360

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 331-360 from the human protein was used as the immunogen for this CDC25B antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CDC25B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CDC25B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy