DNMT3A antibody (AA 463-497)

Catalog No. ABIN3030748

This Rabbit Polyclonal antibody specifically detects DNMT3A in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for DNMT3A antibody (AA 463-497) (ABIN3030748)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DNMT3A Antibody

Binding Specificity: AA 463-497

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Chicken

Purification: Antigen affinity purified

Immunogen: This Dnmt3a antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 463-497 amino acids from the central region of human Dnmt3a.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

UniProt: Q9Y6K1