FBXW7 antibody (N-Term)

Catalog No. ABIN3030899

The Rabbit Polyclonal anti-FBXW7 antibody has been validated for WB and ELISA. It is suitable to detect FBXW7 in samples from Human.

Quick Overview for FBXW7 antibody (N-Term) (ABIN3030899)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FBXW7 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity purified

Immunogen: This FBXW7 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 177-208 amino acids from the N-terminal region of human FBXW7.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the FBXW7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the FBXW7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7

Background: Substrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. [UniProt]

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway