FBXW7 antibody (AA 251-300)

Catalog No. ABIN6747028

Product Details anti-FBXW7 Antibody

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Binding Specificity: AA 251-300

Reactivity: Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Mouse FBXW7

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%) Human, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Zebrafish (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of mouse Fbxw7 (Q8VBV4, NP_536353). Percent identity by BLAST analysis: Mouse, Rat, Hamster, Opossum (100%), Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Zebrafish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7 / FBW7 (FBXW7 Products)

Synonyms: cdc4 antibody, AGO antibody, CDC4 antibody, FBW6 antibody, FBW7 antibody, FBX30 antibody, FBXO30 antibody, FBXW6 antibody, SEL-10 antibody, SEL10 antibody, hAgo antibody, hCdc4 antibody, 1110001A17Rik antibody, Cdc4 antibody, Fbw7 antibody, Fbwd6 antibody, Fbx30 antibody, Fbxo30 antibody, Fbxw6 antibody, F-box and WD repeat domain containing 7 antibody, F-box and WD-40 domain protein 7 antibody, Fbxw7 antibody, FBXW7 antibody, fbxw7 antibody

Background: Name/Gene ID: FBXW7
Family: F-Box

Synonyms: FBXW7, AGO, Archipelago homolog, Archipelago, CDC4, F-box protein FBW7, FBXW6, F-box protein FBX30, F-box protein SEL-10, HCdc4, Homolog of C elegans sel-10, FBW6, FBX30, FBW7, HAgo, SEL-10, SEL10

Gene ID: 55294

NCBI Accession: NP_536353

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway