FBXW7 antibody (C-Term)

Catalog No. ABIN2782955

Cited in 1 publication. The Rabbit Polyclonal anti-FBXW7 antibody (ABIN2782955) specifically detects FBXW7 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for FBXW7 antibody (C-Term) (ABIN2782955)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FBXW7 Antibody

Binding Specificity: C-Term

Purpose: FBXW7 Antibody - C-terminal region

Sequence: LKTGEFIRNL VTLESGGSGG VVWRIRASNT KLVCAVGSRN GTEETKLLVL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against FBXW7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human FBXW7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47419-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXW7 antibody (ABIN2782955)

Gong, Zack, Morris, Lin, Hukkelhoven, Raheja, Tan, Turcan, Veeriah, Meng, Viale, Schumacher, Palmedo, Beroukhim, Chan: "Pan-cancer genetic analysis identifies PARK2 as a master regulator of G1/S cyclins." in: Nature genetics, Vol. 46, Issue 6, pp. 588-94, (2014) (PubMed).

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7

Background:

Background Information: FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.

Gene Name: F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase

Alternative Symbols: AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10, hCdc4, FBXO30, SEL-10

Protein Name: F-box/WD repeat-containing protein 7

Molecular Weight: 80 kDa

Gene ID: 55294

NCBI Accession: NP_001013433

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway