FGFR4 antibody (N-Term)

Catalog No. ABIN3030959

The Rabbit Polyclonal anti-FGFR4 antibody has been validated for WB and ELISA. It is suitable to detect FGFR4 in samples from Human.

Quick Overview for FGFR4 antibody (N-Term) (ABIN3030959)

Target: FGFR4 (Fibroblast Growth Factor Receptor 4 (FGFR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGFR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FGFR4 Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogen: This FGFR4 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 143-178 amino acids from the N-terminal region of human FGFR4.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the FGFR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the FGFR4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for FGFR4

Target: FGFR4 (Fibroblast Growth Factor Receptor 4 (FGFR4))

Alternative Name: FGFR4

Background: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation, MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

UniProt: P22455

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding