HLAG antibody (AA 62-89)

Catalog No. ABIN3031240

This Rabbit Polyclonal antibody specifically detects HLAG in ELISA, FACS, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for HLAG antibody (AA 62-89) (ABIN3031240)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLAG antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HLAG Antibody

Binding Specificity: AA 62-89

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 62-89 from the human protein was used as the immunogen for this HLA-G antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the HLA-G antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HLA-G antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response