HMGB1 antibody (AA 152-180)

Catalog No. ABIN3031204

This Rabbit Polyclonal antibody specifically detects HMGB1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HMGB1 antibody (AA 152-180) (ABIN3031204)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 152-180

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig, Primate, Hamster

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 152-180 from the human protein was used as the immunogen for this HMGB-1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the HMGB-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HMGB-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB-1

Background: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity). [UniProt]

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome