XRCC6 antibody (AA 432-461)

Catalog No. ABIN3029657

The Rabbit Polyclonal anti-XRCC6 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect XRCC6 in samples from Human and Mouse.

Quick Overview for XRCC6 antibody (AA 432-461) (ABIN3029657)

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-XRCC6 Antibody

Binding Specificity: AA 432-461

Purification: Purified

Immunogen: A portion of amino acids 432-461 from the human protein was used as the immunogen for this Ku70 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Ku70 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: Ku70

Background: Ku70 is a single stranded DNA-dependent ATP-dependent helicase. It has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

UniProt: P12956

Pathways: DNA Damage Repair