MCM4 antibody (AA 637-665)

Catalog No. ABIN3031812

This anti-MCM4 antibody is a Rabbit Polyclonal antibody detecting MCM4 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for MCM4 antibody (AA 637-665) (ABIN3031812)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MCM4 Antibody

Binding Specificity: AA 637-665

Cross-Reactivity (Details): Expected species reactivity: Mouse, Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 637-665 from the human protein was used as the immunogen for this MCM4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MCM4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA