Nanog antibody (AA 94-123)

Catalog No. ABIN3031943

The Rabbit Polyclonal anti-Nanog antibody is suitable to detect Nanog in samples from Human. It has been validated for ELISA, IF, IHC and FACS.

Quick Overview for Nanog antibody (AA 94-123) (ABIN3031943)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Nanog Antibody

Binding Specificity: AA 94-123

Cross-Reactivity (Details): Expected species reactivity: Bovine, Primate

Purification: Purified

Immunogen: A portion of amino acids 94-123 from the human protein was used as the immunogen for this NANOG antibody.

Isotype: IgG

Application Details

Application Notes: Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. It is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. [Wiki]

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance