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Retinoblastoma Protein (Rb) antibody (pSer811)

Rb Protein Reactivity: Human ELISA, IHC, IF, DB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3029756
  • Target See all Retinoblastoma Protein (Rb) (Rb Protein) Antibodies
    Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))
    Binding Specificity
    • 15
    • 14
    • 14
    • 14
    • 14
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer811
    Reactivity
    • 174
    • 71
    • 56
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 151
    • 24
    Rabbit
    Clonality
    • 149
    • 26
    Polyclonal
    Conjugate
    • 85
    • 17
    • 10
    • 9
    • 9
    • 9
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    This Retinoblastoma Protein (Rb) antibody is un-conjugated
    Application
    • 89
    • 88
    • 84
    • 27
    • 26
    • 14
    • 14
    • 9
    • 2
    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot (DB)
    Cross-Reactivity (Details)
    Expected species reactivity: Mouse, Rat
    Purification
    Antigen affinity purified
    Immunogen
    This p-Rb1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS811 of human Retinoblastoma.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product Rb Protein Primary Antibody
  • Application Notes
    Titration of the p-Rb1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In 1X PBS, pH 7.4, with 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot the p-Rb1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • Target
    Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))
    Alternative Name
    Retinoblastoma / Rb (Rb Protein Products)
    Background
    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of Rb1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a p-Rb1/HDAC1 repressor complex. Upon calcium influx, Rb1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of Rb1/E2F1 complex thereby disrupting Rb1's activity. [UniProt]
    UniProt
    P06400
    Pathways
    Mitotic G1-G1/S Phases
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