SQSTM1 antibody (AA 317-346)

Catalog No. ABIN3031543

This Rabbit Polyclonal antibody specifically detects SQSTM1 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human and Green Monkey.

Quick Overview for SQSTM1 antibody (AA 317-346) (ABIN3031543)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Green Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 317-346

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 317-346 from the human protein was used as the immunogen for this p62 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the p62 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the p62 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: p62 (SQSTM1)

Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy