PDX1 antibody (AA 1-30)

Catalog No. ABIN3032144

The Rabbit Polyclonal anti-PDX1 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect PDX1 in samples from Human.

Quick Overview for PDX1 antibody (AA 1-30) (ABIN3032144)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PDX1 Antibody

Binding Specificity: AA 1-30

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this PDX1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PDX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: PDX1

Background: PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.

UniProt: O00330

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location