PDX1 antibody (N-Term)

Catalog No. ABIN3032146

This Rabbit Polyclonal antibody specifically detects PDX1 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for PDX1 antibody (N-Term) (ABIN3032146)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDX1 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity purified

Immunogen: This PDX1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 21-55 amino acids from the N-terminal region of human PDX1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PDX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: PDX1

Background: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer, the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location