PDX1 antibody (AA 136-169)

Catalog No. ABIN3032147

The Rabbit Polyclonal anti-PDX1 antibody has been validated for WB and ELISA. It is suitable to detect PDX1 in samples from Mouse.

Quick Overview for PDX1 antibody (AA 136-169) (ABIN3032147)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDX1 Antibody

Binding Specificity: AA 136-169

Purification: Antigen affinity purified

Immunogen: This mouse Pdx1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of mouse Pdx1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Pdx1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Pdx1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: Pdx1

Background: Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer, the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

UniProt: P52946

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location