UCHL1 antibody (AA 16-46)

Catalog No. ABIN3029423

The Rabbit Polyclonal anti-UCHL1 antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect UCHL1 in samples from Human and Mouse.

Quick Overview for UCHL1 antibody (AA 16-46) (ABIN3029423)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-UCHL1 Antibody

Binding Specificity: AA 16-46

Cross-Reactivity (Details): Expected species reactivity: Bovine, Pig

Purification: Purified

Immunogen: A portion of amino acids 16-46 from the human protein was used as the immunogen for this PGP9.5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PGP9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PGP9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: PGP9.5

Background: PGP9.5 is a Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt]

UniProt: P09936

Pathways: Feeding Behaviour