PHGDH antibody (AA 249-277)

Catalog No. ABIN3032220

This anti-PHGDH antibody is a Rabbit Polyclonal antibody detecting PHGDH in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for PHGDH antibody (AA 249-277) (ABIN3032220)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PHGDH Antibody

Binding Specificity: AA 249-277

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Primate

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 249-277 from the human protein was used as the immunogen for this PHGDH antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PHGDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background: 3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect