CDK2 antibody (pThr14)

Catalog No. ABIN3030608

The Rabbit Polyclonal anti-CDK2 antibody is suitable to detect CDK2 in samples from Human. It has been validated for ELISA and DB.

Quick Overview for CDK2 antibody (pThr14) (ABIN3030608)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-CDK2 Antibody

Binding Specificity: pThr14

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Hamster, Xenopus

Purification: Antigen affinity purified

Immunogen: This phospho-CDK2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT14 of human CDK2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-CDK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: Cdk2

Background: CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA