This phospho-E2F1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS332 of human E2F1.
E2F1
Reactivity: Human
WB, ELISA, FACS, IF (p), IF (cc), IHC (p), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Titration of the phospho-E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the phospho-E2F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
E2F1
(E2F Transcription Factor 1 (E2F1))
Alternative Name
E2F1
Background
E2F1 is a member of the E2F family of transcription factors. It binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.