RPS6 antibody (AA 220-249)

Catalog No. ABIN3032477

The Rabbit Polyclonal anti-RPS6 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect RPS6 in samples from Human.

Quick Overview for RPS6 antibody (AA 220-249) (ABIN3032477)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RPS6 Antibody

Binding Specificity: AA 220-249

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Primate, Chicken, Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 220-249 from the human protein was used as the immunogen for this RPS6 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the RPS6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RPS6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly