SMAD1 antibody (AA 1-30)

Catalog No. ABIN3032609

The Rabbit Polyclonal anti-SMAD1 antibody is suitable to detect SMAD1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for SMAD1 antibody (AA 1-30) (ABIN3032609)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMAD1 Antibody

Binding Specificity: AA 1-30

Purpose: SMAD1 Antibody

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Chicken, Zebrafish

Purification: Antigen affinity

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this SMAD1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development