SMAD3 antibody (AA 188-222)

Catalog No. ABIN3032623

This Rabbit Polyclonal antibody specifically detects SMAD3 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for SMAD3 antibody (AA 188-222) (ABIN3032623)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 188-222

Purpose: Smad3 Antibody

Purification: Antigen affinity

Immunogen: This mouse Smad3 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 188-222 amino acids from the Central region of mouse Smad3.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Smad3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Smad3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: Smad3

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). [UniProt]

UniProt: Q8BUN5

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy